Roles & Responsibilities:
Dr. Sahoo’s laboratory conducts research in the field of cardiac regeneration, gene therapy and heart failure to investigate molecular and cellular signaling mechanisms. Current projects involve pre-clinical, translational and basic science research. Exosomes are biological nanovesicles secreted by cells that carry and transfer noncoding RNAs, to mediate local and distant microcommunication between cells, tissues and organs. Her lab was one of the first to study stem cell-derived exosomes and exosomal noncoding RNA-mediated angiogenesis and cardiac regeneration. PMID: 21835908; PMID: 24436429; PMID: 28298297; PMID: 27872050).
Description of the laboratory: Dr. Sahoo’s laboratory studies epitranscriptomic- andexosomes-mediated mechanisms in pathologies and therapeutics of the heart. The projects involve a broad array of advanced techniques in exosomes and epitranscriptome biology, including MeRIP sequencing, bioinformatic data analysis, confocal, super-resolution and electron microscopy, small non-coding RNAs, stable-isotope-labeling mass spectrometry, in vitro and in vivo imaging, live imaging of exosomes, microfluidics isolation, bionanotechnology, systems genetics and small and large animal models of heart failure to address scientific questions in innovative ways.
Position Description: The position is for specialized research professionals who, under the direction of Dr. Sahoo, will provide direct professional support of all research activities in the laboratory. The position involves Bioinformatic data analysis, including, but not limited to small RNA seq data, MeRIP seq data, protein data from mass spec analysis, SILAC experiments, familiarity with processing human, swine and rodent tissue specimens, maintaining an up to date database, managing several laboratory activities and working with other members of the team to guide and provide necessary support as needed. The responsibilities also include maintaining laboratory protocols, safety compliance, grant writing, training new employees, and overall laboratory management.
Preferences would be given to candidates experienced with (1) RNA biology, epigenetics and bioinformatics or (2) mouse models, surgeries, viruses, bioengineering and nanotechnology and (3) with good publication records. We are looking for independent, self-motivated individuals with excellent verbal and written communication skills with a PhD or MD/PhD in Biotechnology/Molecular Biology. The appointment will include a competitive salary and benefits (including Mount Sinai discounted housing for postdocs in NYC), a dynamic multi-disciplinary research environment with access to the Institute’s state-of-the-art facilities that includes in vitro and in vivo imaging, flow cytometry and small and large animal surgical laboratories.
Roles and Responsibilities:
1. Data scientist: Bioinformatic data analysis, including, but not limited to small RNA seq data, MeRIP seq data, protein data from mass spec analysis, SILAC experiments, RNAprotein interaction analysis, undergo training to learn newer methodsw for data handling, processing and analyzing
2. Specialized laboratory technique requirements for the project: Cell and tissue culture, Imaging, flow cytometry, histology of the heart, echocardiography, m6A assay, Western blot, PCR, RNA sequencing and data analysis, exosomes isolation, processing samples for (MeRIP) sequencing, cross linking, and other molecular biology techniques.
3. Other significant laboratory techniques specific for the project: ELISA, transduction, transfection, bacterial transformation, cell proliferation assay, microbial culture, transfection, AAV transduction, miniprep and maxiprep, AAV isolation and purification, IVIS imaging, proficiency in q-PCR, RT-PCR, RNA extraction, cDNA synthesis, DNA/RNA gels, quantification, western blot, immunocytochemistry, fluorescence microscope, agarose gel electrophoresis, spectrophotometer,
4. Animal Handling: Although not a primary part of the responsibility, Sahoo lab projects ivolve animals, and the associate may be required to help other projects and experiemnts with mice handling, simple injections, tissue processing, etc.
Requirements:
The candidate should have a PhD degree with data anlaysis background and some research experience in a laboratory setting in molecular biology, cell culture and animal handling.
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