We are seeking an experienced Scientist in Targeted Proteomics to join our team at Kymera Therapeutics. The successful candidate will develop targeted MS-based assays, characterize their performance and quantify protein targets of interest in preclinical samples across species to assess PD/PK performance of Targeted Protein Degradation drugs. This candidate will be expected to expeditiously generate high quality data, effectively communicate the results to the project teams and collaborate with individuals experienced in each function of drug discovery pipeline and disease area throughout the company.
In 2022, we plan to continue to grow and strengthen our organizational capabilities to deliver on the potential of inventing a new class of protein degrader medicines for patients. Kymera is rapidly advancing three lead programs, with three in clinical-stage and one additional Investigational New Drug Applications (IND) expected by the end of 2022. We are also actively advancing a broad pipeline of preclinical programs across a wide variety of diseases, both internally and in collaboration with our partners Sanofi and Vertex Pharmaceuticals.
In this key role, you will utilize your expertise developing and analyzing data to provide useful results and models in support of our Proteomics team. This is an ideal role for an individual that can excel while multi-tasking in a fast-paced environment. A customer-service mentality is necessary, as you will collaborate with colleagues at Kymera as you join in our efforts to rapidly advance multiple drug candidates to the clinic – promising new therapies designed to treat serious diseases.
Responsibilities:
Develop, optimize, and implement targeted proteomics analysis workflows, including automated antibody enrichment strategies, for precise, accurate, and sensitive protein measurements using stable isotope dilution mass spectrometry. Work with project teams to identify targeted proteomics assay needs, samples requirements and deliverables. Perform targeted protein MS assays using state-of-the-art LC-MS systems; including nano-LC, Orbitrap and TQMS platforms to determine drug PD, optimize SAR, profile E3 ligases and quantify biomarkers for patient stratification. Prepare samples from a wide variety of biological matrices (cultured cells, tumor tissues, biofluids, etc.) Perform data analysis utilizing commercially available or free proteomics software (Skyline, MaxQuant, Spectrum Mill, etc.). Maintain and qualify HPLC and MS instrument performance Keep accurate inventory of peptide standards and reagents Stay up to date in the field of targeted proteomics and mass spectrometry.
Qualifications:
PhD (in Biochemistry, Biology, Chemistry, Chemical Biology, or related discipline) with 3+ years of experience in targeted mass spectrometry-based proteomics in industry or startup biotech setting or BS/MS with 8 + years of relevant proteomics experience Broad experience in targeted proteomics is required for: configuring highly multiplexed and sensitive PRM/MRM stable isotope labeled peptide assays using label-free and TMT-labeled Discovery Proteomics data developing and optimizing targeted MS methods to monitor protein or small molecule targets across a wide dynamic range in a variety of sample types (cells, tissues, biofluids) and formulations (fresh, frozen, FFPE) incorporating antibody or IMAC enrichment strategies of proteotypic peptides or phosphopeptides to improve assay LOD/LLOQ and throughput using LC-MRM-MS acquiring and searching discovery proteomics data to rank and select the best proteotypic peptides to generate targeted protein assays for human and pre-clinical species Operating, maintaining and troubleshooting HPLC, nanoLC, Orbitrap and QQQ instruments Demonstrated ability to independently analyze, and interpret targeted proteomics data to recommend project next steps Ability to organize and multitask efficiently and generate high quality MS data to meet key clinical timelines Ability to communicate data and methodologies effectively in a fast paced, team-oriented environment Working knowledge of operation of lab automation, KingFisher, AssayMAP Bravo or other liquid handling robotic systems is a plus.
Kymera Therapeutics is proud to be an equal opportunity employer, seeking to create a welcoming and diverse environment. All applicants will receive consideration for employment without regard to race, color, religion, gender, gender identity or expression, sexual orientation, national origin, genetics, disability, age, veteran status or any other applicable legally protected characteristics.