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Digambar Gokhale

NCIM Resource Centre
National Chemical laboratory

Publications

2. Gokhale, D.V.,Eun Soo-Han, Srinivasan, S.R. & Deobagkar,D.N. (1984). Transfer of DNA coding for cellulase from Cellulomonas species to Bacillus subtilis by protoplast fusion. Biotechnol. Lett. 6 : 627-632.



3. Gokhale, D.V., Puntambekar, U.S., Vyas, A.K., Patil, S.G. & Deobagkar, D.N. (1984). Hyper-production of -glucosidase by Aspergillus sp. Biotechnol. Lett. 6 : 719-722.



4. Gokhale,D.V., Puntambekar, U.S. & Deobagkar, D.N. (1986). Xylanase and -xylosidase production by Aspergillus niger NCIM 1207. Biotechnol. Lett. 8 : 37-38.



5. Patil, S.G., Gokhale, D.V. & Patil, B.G. (1986). Enhancement in ethanol production from cane molasses by skim milk supplementation. Enzyme Microb. Technol. 8 : 481-484.



6. Gokhale, D.V., Rao, B.S. & Sivaramakrishnan,S. (1986). Alcohol dehydrogenase and invertase activities in ethanol tolerant yeasts. Enzyme Microb. Technol. 8: 623-627.



7. Gokhale,D.V., Puntambekar, U.S., Deobagkar, D.N. & Peberdy, J.F. (1988). Production of cellulolytic enzymes by mutants of A. niger NCIM 1207. Enzyme Microb. Technol. 10 : 442-445.



8. Das, A., Gokhale, D.V. & Peberdy, J.F. (1989). Protoplast fusion and genetic recombination in intra- andinterspecies crossing in Aspergillus niger. Enzyme Microb. Technol. 11 : 2-5.



9. Patil, S.G., Gokhale, D.V. & Patil, B.G. (1989). Novel supplements enhance the ethanol production in cane molasses fermentation by recycling yeast cells. Biotechnol. Lett. 11 : 213-216.



10. Raghav, R., SivaRaman, H., Gokhale, D.V. & Rao, B.S. (1989). Ethanolic fermentation in cane molasses by a highly flocculent yeast. Biotechnol. Lett. 11 : 739-744.



11. Gokhale, D.V. & Deobagkar, D.N. (1989). Differential expression of xylanases and endoglucanases in the hybrid derived from intergeneric protoplast fusion between Cellulomonas sp. and Bacillus subtilis. Appl. Environ. Microbiol. 55 : 2675-2680.



12. Gokhale, D.V. & Deobagkar, D.N. (1990). Secretion of thermostable -glucosidase by an intergeneric bacterial hybrid between Cellulomonas sp. and Bacillus subtilis. Appl. Biochem. Biotechnol. 26 : 207-216.



13. Gokhale, D.V., Patil, S.G. & Bastawde, K.B. (1991). Optimization of cellulase production by Aspergillus niger NCIM 1207. Appl. Biochem. Biotechnol. 30: 99-109.



14. Sathe, S., SivaRaman, H. & Gokhale, D.V. (1992). Protoplast fusion in Yeast : Strain improvement in Saccharomyces. Indian J. Microbiol. 32 : 15-27.



15. Gokhale, D.V. (1992). Fungal protoplast fusion : A tool for breeding industrial strains. J. Sci. Ind. Res. 51 : 497-506.



16. Gokhale, D.V., Patil, S.G. & Bastawde, K.B. (1992). Protection of Aspergillus niger cellulases by urea during growth on glucose or glycerol supplemented media. Appl. Biochem. Biotechnol. 37 : 11-17.



17. Puntambekar, U.S., Gokhale, D.V. & Ranjekar, P.K. (1992). Efficient protoplast regeneration of Bacillus thuringiensis and Agrobacterium tumefaciens. Biotechnol. Techniques 6 : 473-476.



18. Kalkote, U.R., Joshi, R.R., Joshi, R.A., Ravindranathan, T., Bastawde, K.B., Patil, S.G., & Gokhale, D.V. (1992). An efficient enantioselective preparation of 4-(S)-hydroxy-1-(R)-acetoxy-cyclopent-2-ene using new yeast isolate. Biotechnol. Lett.14: 785-788.



19. Gokhale, D.V., Puntambekar, U.S. & Deobagkar, D.N. (1993). Protoplast fusion : A tool intergeneric gene transfer in bacteria. Biotechnol. Adv. 11 : 199-217.



20. Bastawde, K.B., Puntambekar, U.S. & Gokhale, D.V. (1994). Optimization of cellulase free xylanase production by a novel yeast strain. J. Ind. Microbiol. 13 : 220-224.



21. Gokhale, D.V. & Deobagkar, D.N. (1994). Isolation of intergeneric hybrids between Bacillus subtilis and Zymomonas mobilis and the production of thermostable amylase by hybrids. Biotechnol. Appl. Biochem. 20 : 109-116.



22. Patil, S.G., Patil, B.G., Bastawde, K.B. & Gokhale, D.V. (1995). Supplementation of skim milk enhances the cellulolytic activity of fungi. Biotechnol. Lett. 17 : 631-634.



23. Javdekar, V.S., SivaRaman, H. & Gokhale, D.V. (1995). Industrial strain improvement : Construction of flocculent killer yeast by protoplast fusion. J. Ind. Microbiol. 15 : 94-102.



24. Kumar, N.M., Bhide, A., Gokhale, D.V. & Deobagkar, D.N. (1995). Production of extracellular amylase by Cellulomonas sp. NCIM 2353. Biotechnol. Appl. Biochem. 22: 345-354.



25. Gokhale, D.V., Bastawde, K.B., Patil, S.G., Kalkote, U.R., Joshi, R.A., Joshi, R.R., Ravindranathan, T., Gaikwad, B.G., Jogdand, V.V. & Nene, S. (1996). Chemoenzymatic synthesis of D(-)phenylglycine using hydantoinase of Pseudomonas desmolyticum resting cells. Enzyme Microb. Technol. 18 : 353-357.



26. Gokhale, D.V., Patil, S.G. & Bastawde, K.B. (1998). Potential application of yeast cellulase-free xylanase in agrowaste materials to remove hemicellulose fractions. Bioresource Technol. 63 : 187-191.



27. Sudge, S.S., Bastawde, K.B., Gokhale, D.V., Kalkote, U.R. & Ravindranathan, T. (1998). Production of D-hydantoinase by halophilic Pseudomonas sp. NCIM 5109. Appl. Microbiol. Biotechnol. 49 : 594-599



28. Patil, B.G., Bastawde, K.B., Puntambekar, U.S., Gokhale, D.V., Patil, S.G. & Ranjekar, P.K. (1998). Use of tamarind waste to improve ethanol production from cane molasses. J. Ind. Microbiol. Biotechnol. 2: 307-310.



29 Ghorpade, S.R., Bastawde, K.B., Gokhale, D.V., Shinde, P.D., Mahajan, V.A., Kalkote, U.R. & Ravindranathan, T. (1999). Enzymatic kinetic resolution studies of racemic 4-hydroxycyclopent-2-en-1-one using Lipozyme IM. Tetrahedron Asymmetry 10: 4115-4122.



30. Kalkote, U.R., Ghorpade, S.R., Joshi, R.R., Ravindranathan, T., Bastawde, K.B. & Gokhale, D.V. (2000). A practical and scalable process for 4-(R)-hydroxycyclopent-2-en-1-(S)-acetate by desymmetrization of meso-cyclopent-2-en-1,4-diacetate catalyzed by Trichosporon beigelii NCIM 3326, a cheap biocatalyst. Tetrahedron Asymmetry 11: 2965-2970.



31. Mahadik, N.D., Bastawde, K.B., Puntambekar, U.S., Khire, J.M. & Gokhale, D.V. (2002). Production of acidic lipase by Aspergillus niger NCIM 1207 in solid state fermentation. Process Biochemistry. 38(5): 715-721.



32. Galgali, P., Varma, A.J., Puntambekar, U.S. & Gokhale, D.V. (2002). Towards biodegradable polyolefins: strategy of anchoring minute quantities of monosaccharides and disaccharides onto functionalized polystyrene, and their effect on facilitating polymer biodegradation. Chem. Commun. 2002: 2884-2885



33. Mahadik, N.D., Bastawde, K.B., Puntambekar, U.S., Khire, J.M. & Gokhale, D.V. (2004). Production of acidic lipase by mutant of Aspergillus niger NCIM 1207 in submerged fermentation. Proc. Biochem. 39: 2031-2034.



34. Galgali, P., Puntambekar, U.S., Gokhale, D.V. & Varma, A.J. (2004). Fungal degradation of carbohydrate linked polystyrenes. Carbohydrate Polymers 55: 393-399.



35. Adsul, M.G., Ghule, J.E., Singh, R., Shaikh, H., Bastawde, K.B., Gokhale, D.V., & Varma, A.J. (2004). Polysaccharides from bagasse: Applications in cellulase and xylanase production. Carbohydrate Polymers 57: 67-72.



36. Gokhale, D.V., Bastawde, K.B., Puntambekar, U.S., Khire, J.M., Godbole, D.A., Sudge, S.S., Roy Choudhuri, S.K., Kotwal, S.M. & Deshpamde, P.S. (2004). Publication of NCIM catalogue of strains (Third Edition).



37. Singh, R., Singh, S., Trimukhe, K. D., Pandare, K. V., Bastawde, K. B., Gokhale, D. V. & A. J. Varma. (2005). Lignin-carbohydrate complexes from sugarcane bagasse: preparation, purification, and characterization. Carbohydrate Polymer 62: 57-66.



38. Adsul, M.G., Ghule, J.E., Singh, R., Shaikh, H., Bastawde, K.B., Gokhale, D.V., & Varma, A.J. (2005). Enzymatic hydrolysis of delignified bagasse polysaccharides. Carbohydrate Polymer 62: 6-10.



39. Kadam, S.R., Patil, S.S., Bastawde, K.B., Khire, J.M. & Gokhale, D.V. (2006). Strain improvement of Lactobacillus delbrueckii NCIM 2365 for lactic acid production. Process Biochemistry 41: 120-126.



40. Gote, M.M., Khan, M. I, Gokhale, D. V., Bastawde, K. B. & Khire J. M. (2006). Single step purification, characterization and substrate specificity of thermostable alpha-galactosidase from Bacillus stearothermophilus NCIM 5146. Process Biochemistry 41: 1311-1317.



41. Patil, S.S., Kadam, S.R., Bastawde, K.B., Khire, J.M. & Gokhale, D.V. (2006). Production of lactic acid and fructose from media with cane sugar using mutant of L. delbrueckii NCIM 2365. Lett. Appl. Microbiol. 43: 53-57.



42. Adsul, M.G., Bastawde, K.B., Varma, A.J. & Gokhale, D.V., (2007). Strain improvement of Penicillium janthinellum NCIM 1171 for cellulase production. Bioresource Technology 98: 1467-1473.



43. Adsul, M.G., Varma, A.J. & Gokhale, D.V. (2007). Lactic acid production from waste sugarcane bagasse derived cellulose. Green Chemistry 9: 58-62.



44. Adsul, M.G., Khire, J.M., Bastawde, K.B. & Gokhale, D.V. (2007). Lactic acid production from cellobiose and cellotriose by Lactobacillus delbrueckii mutant Uc-3 Appl. Environment. Microbiol. 73: 5055 – 5057.







45. Trimukhe, K.D., Bachate, S., Gokhale, D.V. & Varma, A.J. (2007). Metal complexes of crosslinked chitosans: Part II-An investigation of their hydrolysis to chitooligosaccharides using chitosanase. Int. J. Biological Macromolecules 41: 491-496.



46. Dumbrepatil, A., Adsul, M.G., Chaudhari, S., Khire, J.M. & Gokhale, D.V. (2008). Utilization of molasses sugar for lactic acid production by Lactobacillus delbrueckii subspecies delbrueckii mutant Uc-3 in batch fermentation. Appl. Environ. Microbiol. 74: 333-335



47. K.D.Trimukhe, K.D., Mahadik, N., Gokhale, D.V. & Varma, A.J. (2008). Environment friendly crosslinked chitosan as a matrix for selective adsorption and purification of lipase of Aspergillus niger. Int. J. Biological Macromolecules. 43: 422-425.



48. Mhetras, N., Bastawde, K.B. & Gokhale, D.V. (2009). Purification and characterization of acidic lipase from Aspergillus niger NCIM 1207. Bioresour. Technol. 100: 1486-1490.



49. Adsul, M., Bastawde, K.B. and Gokhale, D.V. (2009). Biochemical characterization of two xylanases from yeast Pseudozyma hubeiensis producing only xylooligosaccharides. Bioresour. Technol. 100: 6488-6495.



50. Adsul, M.G., Terwadkar, A.P., Varma, A.J. and Gokhale, D.V. (2009). Cellulases from Penicillium janthinellum mutants: Solid-state production and their stability in ionic liquids. Bioresources 4: 1669-1680.



51. Gaikaiwari, S.A. and Gokhale, D.V. (2009). White Biotechnology: An effective innovation and sustainable growth. Chem. Ind. Digest November 2009: 86-93.



52. Singhvi, M., Joshi, D., Gaikaiwari S. & Gokhale, D.V. (2010) Protoplast formation and regeneration in Lactobacillus delbrueckii Indian J. Microbiol. 50: 97-100



53. Joshi, D.S., Singhvi, M.S., Khire, J.M. & Gokhale, D.V. (2010). Strain improvement of Lactobacillus lactis NCIM 2368 for D-lactic acid production. Biotechnol. Lett. 32: 517-520.



54. Singhvi, M., Joshi D., Adsul, M, G., Varma, A. J. and Gokhale, D.V. (2010). D(-) lactic acid production from cellobiose and cellulose by Lactobacillus lactis mutant RM2-24. Green Chemistry 12: 1106-1109.



55. Anil H. Valekar, Chandrashekhar M. Malba, Mukund G. Adsul, Digambar V. Gokhale, and Anjani J. Varma. (2010) Organic solvent facilitates acid hydrolysis of pretreated sugarcane bagasse polysaccharides for production of ethanol. Trends in Carbohydrate Research. 2: 10-14.

56. Mhetras, N., Patil, S. & Gokhale, D.V. (2010). Lipase of Aspergillus niger NCIM 1207: A potential biocatalyst for synthesis of isoamyl acetate. Ind. J. Microbiol. 50: 432-437.



57. Adsul, M.G., Rey, D.A. and Gokhale, D.V. (2011). Combined strategy for the dispersion/dissolution of single walled carbon nanotubes and cellulose in water. J. Mater. Chem. 21: 2054-2056.



58 Adsul, M.G., Singhvi, M.S., Gaikaiwari, S.A. and Gokhale, D.V. (2011). Development of biocatalysts for production of commodity chemicals from lignocellulosic biomass. Bioresour. Technol. 102: 4304-4312.



59 Khisti, U.K., Bastawde, K.B. and Gokhale, D.V. (2011). Hyper-production of β-glucosidase and β-xylosidase by Aspergillus niger NCIM 1207 in xylan containing media. BioResources 6: 2066-2076.



60. Singhvi, M.S., Adsul, M.G. and Gokhale, D.V. (2011). Comparative production of cellulases by mutants of Penicillium janthinellum NCIM 1171 and its application in hydrolysis of Avicel and cellulose. Bioresour. Technol. 102: 6569-6572.



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